LOCUS BI553338 722 bp mRNA linear EST 05-SEP-2001
DEFINITION 603193475F1 NIH_MGC_95 Homo sapiens cDNA clone IMAGE:5264635 5',
mRNA sequence.
ACCESSION BI553338
VERSION BI553338.1
DBLINK BioSample: SAMN00163099
KEYWORDS EST.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 722)
CONSRTM National Institutes of Health, Mammalian Gene Collection (MGC)
TITLE NIH-MGC EST Sequencing Project
JOURNAL Unpublished
REMARK http://mgc.nci.nih.gov/
COMMENT Contact: Robert Strausberg, Ph.D.
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Miklos Palkovits, M.D., Ph.D.
cDNA Library Preparation: Michael J. Brownstein (NHGRI), Shiraki
Toshiyuki and Piero Carninci (RIKEN)
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Incyte Genomics, Inc.
Clone distribution: MGC clone distribution information can be
found through the I.M.A.G.E. Consortium/LLNL at:
http://image.llnl.gov
Plate: LLAM11667 row: e column: 20
High quality sequence start: 3
High quality sequence stop: 718.
FEATURES Location/Qualifiers
source 1..722
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="IMAGE:5264635"
/tissue_type="hippocampus"
/clone_lib="SAMN00163099 NIH_MGC_95"
/lab_host="DH10B"
/note="Organ: brain; Vector: pBluescriptR (modified
pBluescript KS+); Site_1: BamHI; Site_2: SalI-XhoI
(gtcgag); Oligo-dT primed using primer
5'-TTTTTTTTTTTTTTTTVN-3', size-selected for average insert
size 2.5 kb and normalized to ROT 5. This is a primary
library enriched for full-length clones and constructed
using the Cap-trapper method (Carninci, in preparation).
Library constructed by M. Brownstein (NIMH/NHGRI, National
Institutes of Health). Note: this is a NIH_MGC Library."
BASE COUNT 214 a 154 c 168 g 186 t
ORIGIN
1 gagcggtccg acggtgtgga agcggacctg aggagtcgca attgtgaaca ggctcagcga
61 tatgaggcag catcgacaat ttatggaccg cacgcattat ctgcttacat tcagctcttc
121 agaaaccttg ctaaggctat tgctacggac acggtagcca acctgagcag aggtccagaa
181 cctccctttt tcaaacaatt aatagttcca ttaattccta gtattgtgga tagagcacca
241 aaaggcagaa ctttcgggga tgtcctgcag ccagcaaaac gtgaatacag agtgggggaa
301 gttgctgaag ttatattgta ggtgctaacc cgaagaattc agtacaaaac cagacccatc
361 agaccttcct cactgtggag aaatatgagg ctacttcaac atcgtggcag atagtgtgta
421 atgatgcctc ctgggagact cgtttttatt ggcacaaggg actcctgggt ctgagtaatg
481 caacagtgga atggcatatt ccagacactg cccagcctgg aatctacaga ataagatatt
541 ttggacacaa tcggaagcag gacattctga agcctgctgt catactttca tttgaaggca
601 cttccccggc ttttgaagtt gtaactattt agtgaaaagt tgatagatcc atttaaagaa
661 cagctttact ctctacacat tatataagtg atttcaaatg aatgtgaact agtgaactac
721 ca
//