LOCUS BI465118 807 bp mRNA linear EST 11-JAN-2011
DEFINITION 603207739F1 NIH_MGC_97 Homo sapiens cDNA clone IMAGE:5273648 5',
mRNA sequence.
ACCESSION BI465118
VERSION BI465118.1
DBLINK BioSample: SAMN00163101
KEYWORDS EST.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 807)
CONSRTM National Institutes of Health, Mammalian Gene Collection (MGC)
TITLE NIH-MGC EST Sequencing Project
JOURNAL Unpublished
REMARK http://mgc.nci.nih.gov/
COMMENT Contact: Robert Strausberg, Ph.D.
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Miklos Palkovits, M.D., Ph.D.
cDNA Library Preparation: Michael J. Brownstein (NHGRI), Shiraki
Toshiyuki and Piero Carninci (RIKEN)
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Incyte Genomics, Inc.
Clone distribution: MGC clone distribution information can be
found through the I.M.A.G.E. Consortium/LLNL at:
http://image.llnl.gov
Plate: LLAM11690 row: m column: 09
High quality sequence stop: 700.
FEATURES Location/Qualifiers
source 1..807
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="IMAGE:5273648"
/clone_lib="SAMN00163101 NIH_MGC_97"
/lab_host="DH10B"
/note="Organ: testis; Vector: pBluescriptR (modified
pBluescript KS+); Site_1: BamHI; Site_2: SalI-XhoI
(gtcgag); Oligo-dT primed using primer
5'-TTTTTTTTTTTTTTTTVN-3', size-selected for average insert
size 2.2 kb and normalized to ROT 5. This is a primary
library enriched for full-length clones and constructed
using the Cap-trapper method (Carninci, in preparation).
Library constructed by M. Brownstein (NIMH/NHGRI, National
Institutes of Health). Note: this is a NIH_MGC Library."
BASE COUNT 220 a 207 c 232 g 148 t
ORIGIN
1 agcggatttc ctcacagttc tagaggctgg gaagtccggg attgaggggc cagcatctac
61 caagggcctt cttgttactt catcccatga tggaaggtgg aagggtaaag agaggagaag
121 aagcagtacc gggaatctta catcagtgac aacctggacc tcgacatgga ccagctggaa
181 aaacggtcgc gggccagcgg gagcagtgcg ggcagcatga aacacaagcg cctgtcccgt
241 cattccaccg ccagccacag cagttcccac acctcgggca ttgaggcaga caccaagccc
301 cgggacacgg ggcagaagac agctactcca gcagtgccat ccaccgcaag ctgaaaacct
361 gcagctcaat gaccagtcat ggcagctccc aaacctcagg ggtggagagt tggcggcaaa
421 gaccggctgg aagaggactt acaggacgat gaaatagaga tgtaggttga tgacccccgg
481 gatctggagc agatgaatga agagtctctg gaagtcagcc cagacatgtg catctacatc
541 acagaggaca tgctcatgtc gcggaagctg aatggacact ctgggttgat tgtgacacga
601 aactgggtct tccacctcga gctccttcag aaaccagttg ttaagcttcg tgggccagag
661 tacattgatt ctcttccaca gactatatgt cgggaaacca aagaaccttc cactggatcg
721 aacaaagttg gagcctcgaa tgaatcacga gtttaccagg aaagacttcc tgggcatgga
781 gggctgtgtc cggacactgg tcacgag
//