LOCUS BG088932 599 bp mRNA linear EST 26-JAN-2001 DEFINITION H3159E02-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3159E02 5', mRNA sequence. ACCESSION BG088932 VERSION BG088932.1 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 599) AUTHORS Kargul,G.J., Dudekula,D.B., Qian,Y., Lim,M.K., Jaradat,S.A., Tanaka,T.S., Carter,M.G. and Ko,M.S.H. TITLE Verification and initial annotation of NIA mouse 15K cDNA clone set JOURNAL Unpublished COMMENT Other_ESTs: H3159E02-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3159 row: E column: 02 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..599 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3159E02-5" /db_xref="taxon:10090" /clone="H3159E02" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 131 a 154 c 151 g 163 t ORIGIN 1 ggcgagttgg tcagtcctct gtgcctgttt cttttgtaaa atgataccta cctcataggg 61 cagttatgag accatatgtg ctcaccttta tttctcaagc atctggaaca gagcctcctg 121 ctgttgctgc tgctgctgct atggaagagc cacgttcgtg tggggagggc tgtgggcccg 181 tttttggagg atgagctaca caggatgagt ctcaggaagc agtccatagt gagcaagggt 241 cagcagctaa ccagaggcgc gtgtcccata atgccctggc gtttttccgc ctttagcact 301 gtagccttaa gtggaagttt cactgctgcc tgtgaactgt tatggcccct cacggcctcc 361 atgcctgtaa tcagggtcac tgtgcataga tccccgtgtc ccacagccca cgctgtgtgc 421 atgtgagagc taagcctttc agtttacttg gtgtctcccc tgtgattcat tgccacacct 481 accccatctg tgtgacagtt tccacccact acagcttcca gtcaaacaat tagtcaatca 541 atgtccgaag ggttttacaa agtagcgaaa ccaaagtaat taatgagtgg agctggcag //