LOCUS BG088910 520 bp mRNA linear EST 12-MAY-2010 DEFINITION H3159C03-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3159C03 5', mRNA sequence. ACCESSION BG088910 VERSION BG088910.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 520) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG088910.1. Other_ESTs: H3159C03-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3159 row: C column: 03 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..520 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3159C03-5" /db_xref="taxon:10090" /clone="H3159C03" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 161 a 90 c 101 g 168 t ORIGIN 1 tgagttgagt ttgtcactgg gctctgctct aattgtggct ttcgttttaa aaaaataaac 61 aacactacat ttgatcacag atgatactga gtatccccaa actactgact tggaagagct 121 agtctccata ctgctcacga gcagtggagc catgttcaaa ttcatcaaag gcaacaagaa 181 gacaatgtga atttttatcg tagaatgtga actgatgtcg taaatgacgc aatgtgaagc 241 ttctgataac acttgtcagg cctcttcctg gtgtcagttt cagtttgtaa aatacgtgtc 301 atgcttcagt tctgcattgt gacaatagtt aaagaaaatg gcacaaaatg tgcatgacca 361 atgggttcgt acgtctgtaa cactgcattg ttccaggtgg acgttatatt gttttcaaaa 421 gtttctcaca atgtatgtta taatattatt actattatat attgtgctca aatgcattct 481 taagagactt ttatatgagc tgaataaact aaagcatgaa //