LOCUS BG088690 695 bp mRNA linear EST 12-MAY-2010 DEFINITION H3156D03-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3156D03 5', mRNA sequence. ACCESSION BG088690 VERSION BG088690.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 695) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG088690.1. Other_ESTs: H3156D03-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3156 row: D column: 03 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..695 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3156D03-5" /db_xref="taxon:10090" /clone="H3156D03" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 152 a 190 c 171 g 182 t ORIGIN 1 gtcccagact gtggccactg agggaggaaa ggggagctcc ttgcacagac agtgaggggc 61 agctcaggac caccttgcat atttagtact cacaggtgaa tgtcacccat gccaggaagg 121 actatcacca ctgggtcaga aacagctctt tgcctccagc ccactcttcc tgtcctcctc 181 tttgttggtc agctgcccct gtggggaggg ctcctgcctt tttttttcag accccccttc 241 tctacccaga tcccacaatc aatgcaaagg gtgctccagg gacttgaggt ggttcctgac 301 ctcccctccc caaaagttca gcctgcccca tgtggcctca ttagggcctt gcaggcctgg 361 tgccaccctg ggggctgcta cttgatggtc ttacccactc atggagtcat ggagttacag 421 gtaccttcct gagcagggga tggtagctcc cgaatactgt aaatgtcact ctcagaggag 481 gaccactgac gggggactgt tacccctagt ctgctgctcc cagggaattt ttttttttaa 541 atacctgtct tccaacaaaa gagtggtcta gatgggggtg gggggctgct aaatttagaa 601 atcatgaccc aaatagcctt ccttttggcc ttttaagttc acaaaagtat tttcaaaatt 661 cccagtgctt ggaagtctaa acttgaagct attcg //