LOCUS BG088556 658 bp mRNA linear EST 12-MAY-2010 DEFINITION H3154F07-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3154F07 5', mRNA sequence. ACCESSION BG088556 VERSION BG088556.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 658) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG088556.1. Other_ESTs: H3154F07-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3154 row: F column: 07 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..658 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3154F07-5" /db_xref="taxon:10090" /clone="H3154F07" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 183 a 140 c 178 g 157 t ORIGIN 1 ggccaaggac aatcctgatt tgtttgcaat gatggagaag acgagaatgt atgttttcag 61 aaacttggat cctgaggaac ccattcagac ctctggatat atttgcaact ttgaggattt 121 ggaaattaag tctgttctct tggatgagat actgaaggac ccagagcacc ctagcaagga 181 ttacataatg aactttgaga tccggtccct gcgagacagc cgagcattga ttgagaaagt 241 tggaattgaa gatgcgtctc agttcataga ggacaaccca cacccccgac tttggcgcct 301 gctggctgaa gctgctctcc agaagctgga cctgtacacc gcacagcaag cgtttgtgcg 361 ctgcaaggat taccaaggca tcaagtttgt gaagctcctg ggcaatctgc agagcgagtc 421 aatgaagcag gctgaagtta ttgcctactt tggcaggttc gaagatgccg aaaggatgta 481 tcaagatatg gacagacggg acctcgctat tggtctcaga atgaaattgg gggactggct 541 cagagtactg cagctcctga aaactggatc tggtgatgca gacgacagtc tccttgaaca 601 agctaacaat gccattggag agtactttgc tgaccgacag aagtggcaga atgctgtg //