LOCUS BG088553 692 bp mRNA linear EST 12-MAY-2010 DEFINITION H3154F02-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3154F02 5', mRNA sequence. ACCESSION BG088553 VERSION BG088553.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 692) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG088553.1. Other_ESTs: H3154F02-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3154 row: F column: 02 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..692 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3154F02-5" /db_xref="taxon:10090" /clone="H3154F02" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 180 a 172 c 158 g 182 t ORIGIN 1 attttccttg gaaacttatg atattacgac agttcgggca tctgtgggca tgtatttaat 61 ttccaagtat attcggaaga acacagacag cgtggtgatc ttctccggag aggggtcaga 121 tgaacttaca cagggctata tatatttcca caaggctcct tcccctgaga aggccgagga 181 ggagagtgag agactgctga aggaactcta cctgtttgat gttctccggg ccgaccgcac 241 tactgctgca cacggtctcg aactgagagt cccctttctg gatcatcggt tttcttccta 301 ttacctgtct ctgccgccag atatgagaat tccaaaaaat ggcatagaaa aacatctcct 361 gagagagact tttgaggact gcaacctgct acccaaagag attctctggc gacccaaaga 421 agccttcagt gatgggatca cctcagtcaa gaactcctgg ttcaagattt tgcaggacta 481 tgttgaacat caggttgatg atgagatgat gtctgcagcc tcccagaagt ttcccttcaa 541 tactcccaaa actaaggaag gctacttcta ccgtcagatc tttgaacgcc attacccagg 601 ccgggctgat tggccgactc attattggat gcctaagtgg atcaatgcta ctgacccttc 661 tgcccgcact ctgacccatt ataagtcagc tg //