LOCUS BG088519 719 bp mRNA linear EST 12-MAY-2010 DEFINITION H3154B03-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3154B03 5', mRNA sequence. ACCESSION BG088519 VERSION BG088519.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 719) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG088519.1. Other_ESTs: H3154B03-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3154 row: B column: 03 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..719 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3154B03-5" /db_xref="taxon:10090" /clone="H3154B03" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 181 a 189 c 192 g 157 t ORIGIN 1 gcggcaggat gaacgagtga tgcagctctt tggcctggtg aacacactcc tagccaatga 61 ccccacttct cttcgcaaga acctcagcat ccagagatac gctgtcatcc ctctgtccac 121 caactcgggc ctcattggct gggtgcccca ctgtgacaca ctgcatgccc tcatccggga 181 ctacagagag aagaagaaga tcctgttgaa catcgagcat cgcatcatgc tgcggatggc 241 tcctgactat gaccacctga cgttgatgca gaaggtagag gtgtttgagc atgctgtcaa 301 caacacagct ggggacgacc tggccaagct actgtggcta aaaagcccca gctcggaggt 361 gtggtttgac cgaagaacca actatacccg ctccctggcc gttatgtcga tggtcggata 421 cattttaggc cttggagaca ggcacccatc caatctgatg ctggaccggc tgagtgggaa 481 gatcctgcac attgactttg gggactgctt tgaggtcgct atgaccagag agaaatttcc 541 agaaaagatt ccatttagac taacaagaat gttgaccaat gctatggagg ttacgggtct 601 ggatggcaac tacagaacca catgccacac cgtgatggaa gtgctccggg aacacaagga 661 cagtgtcatg gctgtgctgg aagcctttgt ctatgaccca ctgctcaact ggaggctga //