LOCUS BG088509 657 bp mRNA linear EST 12-MAY-2010 DEFINITION H3154A01-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3154A01 5', mRNA sequence. ACCESSION BG088509 VERSION BG088509.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 657) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG088509.1. Other_ESTs: H3154A01-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3154 row: A column: 01 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..657 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3154A01-5" /db_xref="taxon:10090" /clone="H3154A01" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 160 a 137 c 203 g 157 t ORIGIN 1 cagcggagcc cggtgacagt tgaggatggc cggagccgag agccccaccg agtgccaggc 61 ggagctggag cccgtggtgt cgctggtgga cgtgctcgag gaagacgagg agttggagaa 121 tgaggcgtgc gccgtcctgg gcggcagcga ctcggagaag tgctcctact cgcagggctc 181 agtggggaga caagcactgt acgcctgcag cacctgcacg ccagagggag aagagcccgc 241 agggatttgt ctagcttgca gttacgaatg tcatggaagc cataaactgt ttgaactata 301 cacaaaaaga aattttcgtt gtgattgtgg aaacagcaag tttaaaaact tggaatgcaa 361 attatttcct gacaaatcaa aggtaaattc ttgcaataag tacaatgaca acttttttgg 421 attatactgc gtttgtaaga gaccttatcc tgatcctgaa gatgaggttc cagatgagat 481 gatacagtgt gtggtctgtg aagactggtt ccatggcagg catcttggtg ccattccccc 541 tgagagtggg gatttccagg agatggtgtg ccaggcttgt atgaggcgct gttctttctt 601 gtgggcttat gctgcccagt tggcagtcac caggatatct gctgaagatg atgggct //