LOCUS BG088477 607 bp mRNA linear EST 12-MAY-2010 DEFINITION H3153F01-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3153F01 5', mRNA sequence. ACCESSION BG088477 VERSION BG088477.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 607) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG088477.1. Other_ESTs: H3153F01-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3153 row: F column: 01 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..607 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3153F01-5" /db_xref="taxon:10090" /clone="H3153F01" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 138 a 133 c 185 g 151 t ORIGIN 1 ggccggggtt ggcgcagttt cgaggcttct tcgcgggcgg cgcttggcgt taactggggc 61 gtggccagga acactccaaa aacagacctg cggctttcac ttctctgttg gtgagaacga 121 gaaggcatca gctaaagttt cagatgcaat ttctactcaa tacccagtgg tggatcatga 181 atttgatgct gtggttgtag gcgctggagg ggcaggcttg cgagctgcat ttggcctttc 241 tgaggcaggg tttaatactg catgccttac aaagctcttt cctacccgat cacatactgt 301 tgcagcacag ggaggtatca atgctgctct ggggaacatg gaagaggaca actggagatg 361 gcatttctat gacactgtga aaggctccga ctggctgggg gatcaggatg ccatccatta 421 catgacagag caagctcctg cctctgtggt tgagctagaa aattatggta tgccatttag 481 cagaactgaa gatgggaaga tttatcagcg tgcatttggt ggacagagcc tcaagtttgg 541 gaaaggcggg caggctcatc ggtgttgctg tgtggctgat cgggcaggcc actcactctt 601 acacacg //