LOCUS BG088273 644 bp mRNA linear EST 12-MAY-2010 DEFINITION H3150H11-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3150H11 5', mRNA sequence. ACCESSION BG088273 VERSION BG088273.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 644) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG088273.1. Other_ESTs: H3150H11-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3150 row: H column: 11 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..644 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3150H11-5" /db_xref="taxon:10090" /clone="H3150H11" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 152 a 179 c 150 g 163 t ORIGIN 1 ggagagatgg ctcagaggtt aggagcactg gctgctcttc ccaaggacca gggtttaatt 61 cccagcaccc acatgacagc tcataactgt ctgtaactcc agtcccaagg gaccggatga 121 tgtcttctgg tctccttgtg catatgtgag ctgcacagac acacaggcag gggacaccta 181 catatgtaaa aattaaacat tgaaaagata aattcacgtg agcttccgag cattgaagga 241 gtcattaaga cttggaccca cctctcacag gtcacagggg ctccagataa gtcctgccta 301 agcctgtcta tctgcctgtc cttgaggctg gggtctctta cctggtcttg ggactccgcc 361 cctcctctag tttccaggac tgtgggcctg aaagtgccaa atgtaagatt ttcctttaag 421 gtcctaaact ttaacttctg tcccgagtct ctctctgtct cagcagatgt ccacttggct 481 gacagacgcc ctccaggaat caactgcgga ctaccaactg ctccaaaaat gactctgcac 541 tctggactgg aggaaagctg atgtgaacca gcccagccga tgtgcttgct ccctgtctct 601 tcagctgggt catctaactg gatgcctctg atgcatgtcc catc //