LOCUS BG088205 648 bp mRNA linear EST 12-MAY-2010 DEFINITION H3149H10-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3149H10 5', mRNA sequence. ACCESSION BG088205 VERSION BG088205.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 648) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG088205.1. Other_ESTs: H3149H10-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3149 row: H column: 10 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..648 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3149H10-5" /db_xref="taxon:10090" /clone="H3149H10" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 160 a 154 c 173 g 161 t ORIGIN 1 gtccttactg ggagttgagg ggaagataca aatggtcgtt tgcatcatca tgggcacacg 61 tgatgatctc tatggagcca tcaagaagct gtgctgcgtg cagtccccag tgccctcaca 121 ggtcatcaat gtccgaacca ttggtcagcc caccaggctt cggagcgtgg ctcagaaaat 181 tttacttcag atgaactgta aactgggtgg tgagctctgg ggagtggata ttccgctgaa 241 acaactaatg gtgattggaa tggatgtgta ccatgacccc agcagaggca tgcgctctgt 301 ggtcggcttc gtggccagca taaatctcac actcaccaaa tggtactcga gggtggtgtt 361 ccagatgcca catcaggaga ttgtggacag cctgaagctc tgcctggtgg gttccttgaa 421 aaagtattat gaggtgaacc attgtctccc agagaaaatt gtggtgtacc gagatggagt 481 gtctgatggc cagctaaaga cagttgccga ctacgagatc cctcagctgc agaagtgttt 541 tgaagccttt gataactacc accccaagat ggtggtgttt gtagttcaga agaaaatcag 601 caccaatctg taccttgctg ctcctgatca cttcgtaacc ccctcccc //