LOCUS BG088181 650 bp mRNA linear EST 26-JAN-2001 DEFINITION H3149F07-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3149F07 5', mRNA sequence. ACCESSION BG088181 VERSION BG088181.1 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 650) AUTHORS Kargul,G.J., Dudekula,D.B., Qian,Y., Lim,M.K., Jaradat,S.A., Tanaka,T.S., Carter,M.G. and Ko,M.S.H. TITLE Verification and initial annotation of NIA mouse 15K cDNA clone set JOURNAL Unpublished COMMENT Other_ESTs: H3149F07-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3149 row: F column: 07 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..650 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3149F07-5" /db_xref="taxon:10090" /clone="H3149F07" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 153 a 180 c 127 g 190 t ORIGIN 1 tagtacctaa catgaacata aattcgatta gctgactgct aactttgtgt ttcttagtta 61 tcctaaacag tttgtaataa cagctttcaa aaggactagg atgtaacatt gcatttttca 121 ttaatatatt tattcacttt acagatcaat tgcagccccg taccctcctc ttttcccaat 181 ctcatcctta caaatctccc ccatcccctc ttcttgggtc caccctgccc tgggacatct 241 ggtccaacag gactaagcac atcctctccc actgaggcca cgcagtccag ttaggggaac 301 ggcatccaat ggcaggcaac cgagtcagag acagcccccg ttccaattgt taggatccca 361 aatgaagacc aatcagcaca tctgctacaa atgtgtaagg gtcgaggacc agccctgcat 421 gctcttcaat tggcggttca gtcctgtaag ccatgcaggc taaggttagt tggttctgta 481 ggtcttcttg cagtgttttt gactcctctg gctcgctcaa ttctatcccc cacccttcca 541 taagctctgc ctgatgtttg gctgtggtct ctacatctgt ttccatcagc tactggatga 601 agcctctcag gagacagttc tgctagtgtc aggggttggc tctcacacat //