LOCUS BG088166 663 bp mRNA linear EST 12-MAY-2010 DEFINITION H3149D12-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3149D12 5', mRNA sequence. ACCESSION BG088166 VERSION BG088166.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 663) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG088166.1. Other_ESTs: H3149D12-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3149 row: D column: 12 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..663 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3149D12-5" /db_xref="taxon:10090" /clone="H3149D12" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 169 a 175 c 176 g 143 t ORIGIN 1 ggatgacgag gcggttgtgg aagtcaacat tccgggcagc gagagcagcg aggccatcgt 61 gcggaaaagg acagctggtg tcctcgacat gggaggcgtg tctacccaga tagcgtacga 121 agtcccccaa actgtaagct ttgcctcctc gcagcaggaa gaagtagcta aaaacctgtt 181 agctgaattc aacctggggt gcgatgtcca ccagactgag catgtgtacc gcgtctacgt 241 ggccacgttt cttgggtttg gtggtaatgc tgcccggcag agatatgaag accgactatt 301 tgccagcaca gttcagaaaa acaggctcct gggtaaacag actggtctga ctcctgatgc 361 tccactactg gatccctgct tgcctctgga cattaaagat gagatccagc aaaacgggca 421 gaccctgtac cttcagggga caggagactt tgacctgtgt cgagagaccc tgcagccctt 481 catgaacaaa accaatgaga cccagacttc cctcaatggc gtctaccagc ctccaatcca 541 cttccagaac agtgaattct acggcttctc tgagttctac tactgcaccg aggatgtctt 601 gcgaatgggg ggagactaca atgctgctag attcactcaa gctgccaagg attactgtgc 661 aac //