LOCUS BG088164 694 bp mRNA linear EST 12-MAY-2010 DEFINITION H3149D10-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3149D10 5', mRNA sequence. ACCESSION BG088164 VERSION BG088164.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 694) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG088164.1. Other_ESTs: H3149D10-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3149 row: D column: 10 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..694 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3149D10-5" /db_xref="taxon:10090" /clone="H3149D10" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 128 a 204 c 201 g 161 t ORIGIN 1 gtgaaaatca cgtctgcagc ggcctctggc ggtgacagct agtattgact ctggggtgtt 61 gctggaatcc tttccaaccg ctggctggag gagcggacat cctcgcagtc ttcggcaata 121 attctcctgg actgtggctg gttatattgg cctaggctct gcagaccaga agggctggaa 181 ctcacatgga acggtgccgg gctggcccgt gccaagcgca tgaagcacct tcatgaccac 241 ctgtggacac aggtgggcag aggatcctct taaccagtct gaaggatggc acacaagaca 301 ctggtcctca ggccctgcta agcacagccc ctcactctcc ttcccggcct gggtgcagac 361 ccagcccctg ccatcctgag aggctccttg gtcttatgac ccctgttctt cagaggcttt 421 ttccaccaaa gccatagctg ggaatggttg tggccccttc ctgcaggggt ggtaagatgg 481 agagaatggt ggggaggccc ctctccagtg aagaaatggc cagtcatctc acccagccct 541 cagaccctcc ttccacgttg gacctgctgt gggcactccg aggctgtggg tagggcctct 601 tagggcttgg tgttttgggg gtacctgccc ctccatcagt gtctgggcat gagttatcac 661 tcatctggct cgtcttggcc tgacactcca gctg //