LOCUS BG088162 677 bp mRNA linear EST 12-MAY-2010 DEFINITION H3149D08-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3149D08 5', mRNA sequence. ACCESSION BG088162 VERSION BG088162.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 677) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG088162.1. Other_ESTs: H3149D08-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3149 row: D column: 08 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..677 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3149D08-5" /db_xref="taxon:10090" /clone="H3149D08" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 237 a 117 c 190 g 133 t ORIGIN 1 attgcgtcca aagaacaaga ctttttcttt gaacttgatg ccatggatca tctgcagtca 61 ttcattgcag attgtgatag aagaacagaa gtatctaaga aaagactagc agaaactcaa 121 gaagagataa gtgctgaagt ggctgcaaag gcagagcgtg ttcatgagtt aaatgaggaa 181 ataggcaagt tgttggccaa agtggaacaa ctaggagctg aaggaaatgt ggaagaatct 241 cagaaagtca tggatgaagt cgagaaagca cgggcaaaga aaagagaagc agaggaagtt 301 tatcggaact ctatgccagc ttccagcttc cagcagcaga aacttcgtgt ctgcgaggtc 361 tgctccgcgt atttaggtct tcatgacaat gacaggcgac tggctgatca ttttgggggt 421 aaattgcacc tgggatttat tgaaataaga gagaagctgg aagaattaaa gagagttgta 481 gctgagaagc aggagaagag aaaccaggaa cgtctgaaac gaagagaaga gagggagaga 541 gaagaacggg agaaactgag gaggtcccga tcacatagca agaatcccaa aagatctaga 601 tccagagagc atcgcagaca ccgatctcgc tccatgtcac gagaacgcaa gaggaggact 661 cgatccaagt ctcgtga //