LOCUS BG088114 663 bp mRNA linear EST 12-MAY-2010 DEFINITION H3148G11-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3148G11 5', mRNA sequence. ACCESSION BG088114 VERSION BG088114.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 663) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG088114.1. Other_ESTs: H3148G11-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3148 row: G column: 11 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..663 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3148G11-5" /db_xref="taxon:10090" /clone="H3148G11" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 146 a 172 c 124 g 221 t ORIGIN 1 tttgatgtgc tcttactgga cactactctt gacttctggg ttggtagtag atctctattt 61 ccagggactg ttcgcctgtt tactcaagag tttctcctgt ggattctaat gcttgtcttc 121 tccagattag tctcattttt ctttctttcc ttaccctccc ttagtctctc cagaaatgat 181 ttcttctcaa gaactgcttc ccaagccatg tgtgtccagt gcagatattt ctaccaagga 241 ttcgttctgc tctctgcagc atcttgtgtt gctactctaa ctcccaaaac atcattttaa 301 aatattccat ccacacagcc tgtcacttta aaacttggat gatttttcta caactggagc 361 ttgagtacta gacttcttat cttgacagcc agagtctggc cttagccatg ctggaggtca 421 gttcttccac ggggttgggc ttttactgcc agggctaact cacaagtcat gtgtaatact 481 tttagcctag aacgcccagt aattctggtt gcactctgtc tgtcccctac caatttctca 541 attcctaggc acagacaaaa ttcccaagcc tggcttatga gtctggactg agcagtatgg 601 cctgactcac gggactggac ggagcatgac tcctttcaaa tcctcagagc acttactgca 661 att //