LOCUS BG087707 658 bp mRNA linear EST 12-MAY-2010 DEFINITION H3142C09-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3142C09 5', mRNA sequence. ACCESSION BG087707 VERSION BG087707.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 658) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG087707.1. Other_ESTs: H3142C09-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3142 row: C column: 09 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..658 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3142C09-5" /db_xref="taxon:10090" /clone="H3142C09" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 169 a 166 c 88 g 235 t ORIGIN 1 cagaattgtt ggcaaaaaca tcccctcacc agcaagatgt ctgctggttt aagcaacttg 61 gtctcttgat gccattagca aaagtattaa ttgtccaaag cacctttgtt cactaatatc 121 tatctatcta tctatctatc tatctatcta tctatctatc tatctatcta tctatcatct 181 atctacctac ctatctacct atcatctatc tatctatcat ctattatcta tctatctatc 241 tatctatcta tctatctatc tatctatcca tctatctatc catcatctat ctacctacct 301 atctactatc catctatcta tctatccatc atctatctac ctacctatct actatccatc 361 catttatcta tctatctatc tatctatcta tctatctatc tccctcatac ttctgagaca 421 tggccagttt tcttccctcc ctgctgttaa gcacttggca gatgaggggg ggggtcccat 481 ttatttctga gtgagatggt gagcaatctg tatgttggct gaaaagaaaa aaaaatctcc 541 agtctgaatt gggaagaaat ctggtctcta agctcagatt ccttgctgta cagaagctgt 601 gtatatacgt agcccgtttc tgaagggtac aggaaggggg cacggctgtc ttcccttc //