LOCUS       BG087696                 636 bp    mRNA    linear   EST 12-MAY-2010
DEFINITION  H3142A04-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
            H3142A04 5', mRNA sequence.
ACCESSION   BG087696
VERSION     BG087696.2
DBLINK      BioSample: SAMN00163148
KEYWORDS    EST.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 636)
  AUTHORS   Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
            Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
            Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
  TITLE     Genome-wide expression profiling of mid-gestation placenta and
            embryo using a 15,000 mouse developmental cDNA microarray
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
   PUBMED   10922068
COMMENT     On Jan 26, 2001 this sequence version replaced BG087696.1.
            Other_ESTs: H3142A04-3
            Contact: George J. Kargul
            Laboratory of Genetics
            National Institute on Aging/National Institutes of Health
            333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
            Email: cdna@lgsun.grc.nia.nih.gov
            This clone set has been freely distributed to the community. Please
            visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
            Plate: H3142  row: A  column: 04
            Seq primer: -21M13 Reverse
            POLYA=No.
FEATURES             Location/Qualifiers
     source          1..636
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6J"
                     /db_xref="niaEST:H3142A04-5"
                     /db_xref="taxon:10090"
                     /clone="H3142A04"
                     /sex="clones arrayed from a variety of cdna libraries"
                     /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
                     /dev_stage="Clones arrayed from a variety of cDNA
                     libraries"
                     /lab_host="DH10B"
                     /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
                     clone is among a rearrayed set of 15,247 clones from 11
                     embryo cDNA libraries (including preimplantation stage
                     embryos from unfertilized egg to blastocyst, embryonic
                     part of E7.5 embryos, extraembryonic part of E7.5 embryos,
                     and E12.5 female mesonephros/gonad) and one newborn ovary
                     cDNA library. Average insert size 1.5 kb. All source
                     libraries are cloned unidirectionally with Oligo(dT)-Not
                     primers. References include: (1) Genome-wide expression
                     profiling of mid-gestation placenta and embryo using a
                     15,000 mouse developmental cDNA microarray, 2000, Proc.
                     Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
                     cDNA analysis reveals phased gene expression patterns
                     during preimplantation mouse develolpment, 2000,
                     Development, 127: 1737-1749; (3) Genome-wide mapping of
                     unselected transcripts from extraembryonic tissue of
                     7.5-day mouse embryos reveals enrichment in the t-complex
                     and under-representation on the X chromosome, 1998, Hum
                     Mol Genet 7: 1967-1978."
BASE COUNT          158 a          191 c          155 g          132 t
ORIGIN      
        1 gcagcccgca gctgatccgg gtgctcagcg gactgcgaga cctgggagcc gggtccggag
       61 ccgatcgaac gccgcgcagc gctggttgag cctctgtagg gagctgctgt gctctccagc
      121 caagcatgct gtggtcagat ctgcccagcc ctggaataga atcctttgcc ggatggaaaa
      181 tccataaaag aaagctcttg tgaagagctg attcccacct gctgacttaa gtgcgcccag
      241 ctagccctct ctctgtttct ggagtccttg ggagttcctg agtcccccac tcacacgctg
      301 actgtcgcta tgggcagaag gtacccagag gctcgagggt gtccaagcag ctcatatctc
      361 aaattataac ctgtttcctg caccattgct gacgtccaac gatccatgtc agaagtactt
      421 ccagctgact caggtgttgg caccttggca gtgtttatgg ccagcagcgg atccacagac
      481 attgcaaacc ggaacagccc agccacacca ccaaataccc tcaatctccg ttcctcccac
      541 aatgaactat taaatgcaga gatcaaacac tcagatgcca agaacagcac gccccccaaa
      601 tgcaggaaaa aatatgcact gactaatatt caggcg
//