LOCUS BG087674 602 bp mRNA linear EST 12-MAY-2010 DEFINITION H3141F09-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3141F09 5', mRNA sequence. ACCESSION BG087674 VERSION BG087674.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 602) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG087674.1. Other_ESTs: H3141F09-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3141 row: F column: 09 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..602 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3141F09-5" /db_xref="taxon:10090" /clone="H3141F09" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 135 a 166 c 188 g 113 t ORIGIN 1 cttgccggca gactcgccgc catgggccgt gtgatccgag ggcagaggaa aggcgcgggt 61 tctgttttcc gtgcgcacgt gaagcaccgt aagggcgccg cgcgcctacg tgctgtggac 121 ttcgcggagc gacacggcta cattaaaggc atcgtaaagg acatcattca tgaccctggc 181 cgcggcgctc ccctcgccaa agtcgtcttt cgggatccct accgattcaa gaagcggaca 241 gagctgttca tcgcagcgga ggggatccac actggacagt tcgtgtactg cggcaagaag 301 gcccagctga atatcggcaa tgttttgccc gtgggcacca tgcctgaggg tacgatcgtg 361 tgttgtctgg aggagaaacc tggggacagg ggcaagctgg cccgagcctc cgggaactac 421 gccacagtca tctcccacaa cccagagacc aagaagactc gagtgaagct gccttcaggg 481 tccaagaagg tcatttcctc tgccaacaga gccgttgttg gtgttgtggc tggtgggggc 541 agaattgaca agcctatctt aaaggctggc cgtgcctacc acaagtacaa ggcaaagagg 601 aa //