LOCUS BG087543 594 bp mRNA linear EST 12-MAY-2010 DEFINITION H3141A05-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3141A05 5', mRNA sequence. ACCESSION BG087543 VERSION BG087543.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 594) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG087543.1. Other_ESTs: H3141A05-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3141 row: A column: 05 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..594 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3141A05-5" /db_xref="taxon:10090" /clone="H3141A05" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 107 a 175 c 164 g 148 t ORIGIN 1 ttttgttgtt gttgttacct gtttgccttc gctcctattc tgtcctattt ctgtactaga 61 gaaagataca ttgtctcccc taccagctcc gctaagctgg tttgatagct cactccctga 121 atcggagttc cttccccgac gcccccatgt agctgagaag tgggacctag gggtggttgg 181 acccctggga tcctgaagga gaggcagagg agggcgcagg actcggcttc tgctcctgcg 241 atcagaacgc gaccttcgca gctttacccc tcccgctgcc atgcaccctg cggccttccc 301 gcttcctgtt gtcgtggcta ctgtactgtg gggagcagcc cctgtccgag ggctgattcg 361 agcgacttcg gagcacaatg ccagcatgga ctttgcagac cttccagctc tgtttggggc 421 cactctgagc gacgagggac tgcaggggtt ccttgtggag gcccacccag aaaatgcctg 481 cggtcctatt gccccaccac cctcagcccc ggtcaatggg tcctaaatgc tcagaaggct 541 gggtatggtg cagctgtggt gcacaatgtg aattccaatg aactgctgaa catg //