LOCUS BG087469 612 bp mRNA linear EST 12-MAY-2010 DEFINITION H3140B01-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3140B01 5', mRNA sequence. ACCESSION BG087469 VERSION BG087469.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 612) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG087469.1. Other_ESTs: H3140B01-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3140 row: B column: 01 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..612 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3140B01-5" /db_xref="taxon:10090" /clone="H3140B01" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 194 a 147 c 150 g 121 t ORIGIN 1 ggttgctaag gagctgccct tcagctgctg gaggacaggg gttcttgatc caactgcgcg 61 atggaacaaa acgcctcaaa accccactac cccacagtgt ccgattttgc ttggtgttag 121 atcctgcttt ttttgtttgg aacgtggaag tgaacttgac tctcatgaag aaccaggagg 181 acccctcaga ggaaatgcca aagacagcca agacccagaa ctaaggaaca agacccttgc 241 ctgcctcagt acttccctgg ctgagtacaa ccagccctct gaggctgaca ccttaaagag 301 catgaaagcc aaagcattca aaaattaaaa agaaatcaga gctaactcca aaaaaagaga 361 aactgggaag aaaaatgaaa aggacacata aagagaggaa tatggagatg gcggcagggc 421 tgagcaagtc agacatcacc aactccaagg aagcaggtgg cacctcacat caagggctcc 481 tccgctccca ctctgcagct ggacaacttt ctcttgaact cgatgctctg gagagtcagg 541 ttgctataga tggaagatta tcatccatcc aggccacaga tgttgctagt gacatggaat 601 gtgaagagga ga //