LOCUS BG087229 709 bp mRNA linear EST 12-MAY-2010 DEFINITION H3137A10-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3137A10 5', mRNA sequence. ACCESSION BG087229 VERSION BG087229.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 709) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG087229.1. Other_ESTs: H3137A10-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3137 row: A column: 10 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..709 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3137A10-5" /db_xref="taxon:10090" /clone="H3137A10" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 164 a 212 c 194 g 139 t ORIGIN 1 ccccaaagtc atcgagtgct tcaatgtgga atcacgcatc ctgtgtatgg tatacattcc 61 cgcagaggag tcaaagcccc aagaaaccac cgaaaccaag gatccagaag ccacagcctc 121 gagagcaccc catgtgccca ccatctgcct gggcacagaa gagggaagca tttccattta 181 caaaagcagt cagggctgca agaaggtgag gcttcagcac ttctatgcac cggacaagtc 241 cacggtcatg agcttggcct gctccccaca aggcctgtat gcaggcctgg tcaatgggtc 301 cgtagccagc tacaccaagg ctccagatgg atcctggaac tcggaacccc agcaagtgat 361 caagttgggt gtcctgccag tcagaagcct tctcctggtg gagggtgcac tgtgggcagc 421 ctcgggaggg caggtgttca tggccagcgt ggagactcac actatagaga atcagctgga 481 ggcccaccaa gatgaaggga tggtcatctc ccacatggct gtggctggcg ttggcatctg 541 gattgccttc acctcggggt ccaccctccg cctcttccac acagagacct tgaagcacct 601 gcaggatgtc aacattgatg cccctgtgca tagcatgctg ccagggcacc agcgtctatc 661 tgtgaccagc ctgctcgtct gccacggctt gctcatggtc ggtaccagc //