LOCUS BG086861 587 bp mRNA linear EST 12-MAY-2010 DEFINITION H3131D12-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3131D12 5', mRNA sequence. ACCESSION BG086861 VERSION BG086861.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 587) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG086861.1. Other_ESTs: H3131D12-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3131 row: D column: 12 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..587 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3131D12-5" /db_xref="taxon:10090" /clone="H3131D12" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 168 a 115 c 126 g 178 t ORIGIN 1 atgatgccat catttaaagt acttcagtgg tcaaggaaag aagaaagact gcagccttat 61 caattatttt ggtttatatt agtttaaact attattttct tggaagtagt ataaacaagt 121 catgcatata atgagcagtg tattccatga atatctggaa atttctcatt actataggta 181 aagggtatag tctgggtaaa atgaatattg ggagctaaca ggtcatttat ccagtgattg 241 catttccttt agcaagacaa gtcctatctt ttggaatatg tgcatggcat tgattggtca 301 tgtaggacca acatcactga gtagcagggc ccatctgcat acaggtgagg agcctgtcac 361 agcatcccgg atctcagtat cttctctgct cacataaagc acttgagtgc cagtcagagc 421 tgggtgatcc taattcctgc ctcaggttcc atggttgcgc taaccagctt tcaagtgcgg 481 ttgaagtaag tttcagtatt ttagggttta gattagagtt catctagaag tagaacttgc 541 agtccaaata catcccccac gacctcctat gctaacggcc accgagc //