LOCUS BG086686 550 bp mRNA linear EST 26-JAN-2001 DEFINITION H3129D01-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3129D01 5', mRNA sequence. ACCESSION BG086686 VERSION BG086686.1 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 550) AUTHORS Kargul,G.J., Dudekula,D.B., Qian,Y., Lim,M.K., Jaradat,S.A., Tanaka,T.S., Carter,M.G. and Ko,M.S.H. TITLE Verification and initial annotation of NIA mouse 15K cDNA clone set JOURNAL Unpublished COMMENT Other_ESTs: H3129D01-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3129 row: D column: 01 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..550 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3129D01-5" /db_xref="taxon:10090" /clone="H3129D01" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 149 a 101 c 162 g 138 t ORIGIN 1 agagattgat ttctggagag agattcatgc atgatgttga aaggttcctg aataaactgc 61 tttgagaaga acgcagtgtt gtcgctcttt cctgctggtc agaataggaa gctacaaatg 121 gtggcccgta cggggaccct tctcgacgtt cagaactcat ctcagtcagg gcagctagct 181 tgtaaggttc ccaggtaagt gggacaattt aagttctcgg attgagacga taaggttccc 241 agttttggga caagttaagg ttcccggttt tgggacatgt tgcaccaagc accatgggga 301 cttgaggttt gcagaggaac agagggtttt actaaaaggg gttttgccgg tctggaagtt 361 agtgagaaat tgcatagaag ataaagatgt gacgtagaac tccagagagg taatgaggcg 421 ctgagtcacg tgagagagga acactctatt ggtttgggcg agagggtctg tttgtttcct 481 caggatcacc gacaacagct gtgggtccca gaaccgctga ccagagcaat tcaaaacaag 541 cagagctatg //