LOCUS BG086558 693 bp mRNA linear EST 12-MAY-2010 DEFINITION H3127G04-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3127G04 5', mRNA sequence. ACCESSION BG086558 VERSION BG086558.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 693) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG086558.1. Other_ESTs: H3127G04-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3127 row: G column: 04 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..693 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3127G04-5" /db_xref="taxon:10090" /clone="H3127G04" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 197 a 153 c 196 g 147 t ORIGIN 1 aatgatagtc acaagaaagc tgctcaccat caaaagcaag ccgcttttat tcagacccat 61 catggagtta cacccagagg acacgcttca aatggatagt cacatgctgg attcaaaaag 121 atatgccatc attggagcag atctccgaga cctttctgag ctggaagaga aactaaagaa 181 atgtaacatg aatacacaat tgccaacact cctgataact gaatgtgtgc tggtttacat 241 gactccagag cagtcagcca acctcctgaa atgggcagcc agcagttttg agacagccat 301 gttcataaac tatgagcagg tcaacatgga cgaccggttt gggcagatca tgattgaaaa 361 cctccggagg cgaagttgtg acctggcagg agtggaaacc tgcaaatcac tggagtccca 421 gaaggaacgg ctgctgttga atgggtggga gacagcgtct gcagtgaaca tgatggagct 481 gtacagtggg ctacctcggg cagaggtaaa cagaatagaa tccctcgagt tcctggatga 541 gatggagctc ctggagcagc tcatgcgcca ttactgcctg tgctgggcca cccgcggcgg 601 ccaggagctt ggtttgaagg agataaccta ttgatctgct gaaggctcgt gctggggtgg 661 aaggtgaagg ctctagctaa gggagtgtca ggg //