LOCUS BG086531 685 bp mRNA linear EST 12-MAY-2010 DEFINITION H3127D05-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3127D05 5', mRNA sequence. ACCESSION BG086531 VERSION BG086531.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 685) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG086531.1. Other_ESTs: H3127D05-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3127 row: D column: 05 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..685 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3127D05-5" /db_xref="taxon:10090" /clone="H3127D05" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 168 a 171 c 174 g 172 t ORIGIN 1 ctccggagga acctggctca ccggccgagg tcttgccgtg agcccttgtg tgtacagtag 61 caggctgtaa ctgagacaga cagctgcagt gcttacagac acataatgtc aatagtccgt 121 tcatccgtcc acgccaaatg ggttgtgggg aaggtgattg gtacagccat gataaaaacg 181 gctaaagtaa gagcgaccag acttgttttg gatccctact tactgaagta ctttaataaa 241 cggaaaacct actttgctca cgatgccctt cagcagtgca gcgtcgggga catcgtgctc 301 ctcagagctt tgcccgtccc acgatcgaag catgtgaagc acgaactggc tgagatcatc 361 ttcaaagtcg gccgagttat cgaccccgtc accggaaagc cctgtgcagg aactgcctac 421 ctagagagcc ccctcagcga acctcgggag gagctcaagg tctcctgaac ccaggaaagg 481 aggagccaga ggcatttctg tctgtgtgtt ttgctcaatg gttttcatta ctgtttctcc 541 tgtgacatta aaagtagtca gcaaagtaaa gagcttagtg ctccatacag ggttgcatcc 601 tttttcagat tctctgaact gaaggtactg attactctgt atgcctaagg atgcggcatc 661 attttgtgag ctctctgtcc tggat //