LOCUS BG086523 656 bp mRNA linear EST 12-MAY-2010 DEFINITION H3127C07-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3127C07 5', mRNA sequence. ACCESSION BG086523 VERSION BG086523.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 656) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG086523.1. Other_ESTs: H3127C07-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3127 row: C column: 07 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..656 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3127C07-5" /db_xref="taxon:10090" /clone="H3127C07" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 120 a 198 c 209 g 129 t ORIGIN 1 cggcgctgcg agaggtcctc cgagatgctg cggttgttcc aggccgcttc ccgggcctcg 61 cttcgactct ctggctcccg ggtcatccac tctctagcgg aaggcgcgga gcggccggcc 121 gagatttcag agccgcgaga tagcgctggt ctcctagatc ctgttttgcg caagtgtgag 181 ctccggatcc cagtgcaccg gcgtccggtg caagcctggg tcgagtctct ccgaggcttt 241 gagcaggagc gcatcggcct ggcggagctg caccccgacg tgtttgcaac ggcgcctcgg 301 ctggacattg tgcaccaggt cgccatctgg cagaggaact tcaggagaat cagctatgct 361 aacaccaaga ccagggctga ggtgagtggt ggtggccgca agccctggca acagaaaggc 421 agtggccgtg ctcggcatgg cagcatccgt tctcccttat ggcgaggagg aggtgtagcc 481 catggtcccc ggggtcctac aagttactac tacatgttgc ccatgaaagt gcgggcactg 541 ggcctcaagg tggccttgac tgtcaagctc atgcaggatg atctacacat tgtggattcc 601 ctggagctac ccacggcaga cccccagtac ctgacagagt tggcccagta ccgcca //