LOCUS BG086218 538 bp mRNA linear EST 12-MAY-2010 DEFINITION H3123B09-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3123B09 5', mRNA sequence. ACCESSION BG086218 VERSION BG086218.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 538) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG086218.1. Other_ESTs: H3123B09-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3123 row: B column: 09 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..538 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3123B09-5" /db_xref="taxon:10090" /clone="H3123B09" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 136 a 132 c 137 g 133 t ORIGIN 1 aagtgaactc aatgcataca gtcatgaagt cattaccatc cttggagaga ttgattttat 61 gttggggaat cttcctgaat tggcttctgc gagaccggct aagaagaacc tgctaaccat 121 gatggatgag gcctacgttc agcccgagcc tctgggagtc gtactgatta ttggagcttg 181 gaattaccct ttcgttctta ccatgcaacc gctggtggga gccattgctg caggaaatgc 241 tgccattgtt aagccctcag aactcagtga aaacacggcc aagatcttgg ctgaactcct 301 ccctcagtac ttagaccagg acctgtatgc gattgttaat ggcggtatcc cggaaaccac 361 ggagcttctg aagcagcggt ttgaccacat tctctataca gggaacactg cagttggaaa 421 aattgtcatg gaagctgctg ccaagcacct gacccctgtg accctggaac tcggcgggaa 481 aagcccttgt tacattgaca gagactgtga tctggacgtg gcttgcagac gcatagcc //