LOCUS BG086216 640 bp mRNA linear EST 12-MAY-2010 DEFINITION H3123B06-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3123B06 5', mRNA sequence. ACCESSION BG086216 VERSION BG086216.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 640) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG086216.1. Other_ESTs: H3123B06-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3123 row: B column: 06 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..640 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3123B06-5" /db_xref="taxon:10090" /clone="H3123B06" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 196 a 107 c 163 g 174 t ORIGIN 1 gaggccatgg aactcattat tgctcatgga cgggaaaatg gagctgacag tattttggac 61 ctcggattat tagactcttc agaaaaggta caggaaaaca gaaagcgcca tggctctagt 121 agaagtgtag tggatatgga tttagaggac acagatgatg gtgatgacaa cgctcccttg 181 ttttaccaac ctgggaagag aggattttat actccgagac ctggcaaaaa cacagaagca 241 aggctgaatt gtttcagaaa catcggcagg attcttggac tatgtctgtt gcaaaatgaa 301 ctatgtccta tcacattgaa taggcatgta attaaagtgt tgcttggtag aaaagtcaac 361 tggcatgact ttgctttttt cgaccctgta atgtatgaga gtttacgaca actgatcctt 421 gcatctcaga gttcagatgc tgatgctgtt ttctcagcaa tggatttggc atttgcaatt 481 gacctgtgta aagaagaggg tggaggacag gttgaactta ttccaaatgg tgtaaatata 541 ccagtcactc cgcagaatgt atatgagtat gtaaggaaat atgcagagca tagaatgttg 601 gtggttgcag aacagccatt gcacgcaatg agaaagggtc //