LOCUS BG086162 581 bp mRNA linear EST 12-MAY-2010 DEFINITION H3122E06-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3122E06 5', mRNA sequence. ACCESSION BG086162 VERSION BG086162.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 581) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG086162.1. Other_ESTs: H3122E06-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3122 row: E column: 06 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..581 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3122E06-5" /db_xref="taxon:10090" /clone="H3122E06" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 147 a 126 c 109 g 199 t ORIGIN 1 aggtatgcga ctttcaattt caacatccct aaggaaaaaa aaaatctgta tgcatattgc 61 ttgctgggtc accaccacct cctttattat ttgcatatga ttaagtccct tttagataca 121 tttccatcaa aatgaaaatt tttgaataat gtggctatta ttgcttcatt aaggctggct 181 ctaggattcg acctggagag atgagcttgt aaaagcttcg cctgcaaact tgacctgttt 241 cttgtcgctt ttaatttttg tctttatttt atttaccctc cataataatg ggagtgttaa 301 ctgccagtga ccacctctga ttgtaggtct ttgttcatgg gcaccccccc cccatcttga 361 agaaaagaaa agtgtaaggt ctgtttattt cttattttat taggaataaa tccaagatag 421 gtggtctaag gcagacagtg agggtttgaa atctagcaaa cgctggctat tttctcgctg 481 tctacacggt tcattagcct gtcttgtcag ttttccccgg ccgaacgctt ctagacctca 541 ctgaacattg ctttgtgtgg tactcccttc aagctctccc a //