LOCUS BG086072 644 bp mRNA linear EST 12-MAY-2010
DEFINITION H3121D12-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3121D12 5', mRNA sequence.
ACCESSION BG086072
VERSION BG086072.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 644)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG086072.1.
Other_ESTs: H3121D12-3
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3121 row: D column: 12
Seq primer: -21M13 Reverse
POLYA=No.
FEATURES Location/Qualifiers
source 1..644
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3121D12-5"
/db_xref="taxon:10090"
/clone="H3121D12"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 178 a 147 c 143 g 176 t
ORIGIN
1 agaaccctga agatatgggt gacctctatc ttgatgttgc tgaagctttt ctggatgttg
61 gtgaatataa ttctgccctt cccctgctca gtgccctggt ttgctctgaa agatacaacc
121 tcgcagtggt ttggcttcgg catgcagaat gtttaaaggc cttgggctac atggaacgtg
181 ctgctgaaag ctacagtaaa gtggttgatc tggctccact ccatttggat gcaagaattt
241 cacttcccat cctgcagcag cagctaggcc gtcctgagaa agctctggaa gcgctggaac
301 ccatgtatga tccagacacc ttagcccaag atgccaatgc tgcacagcag gaactgaaat
361 tactgctcca tcgttctact ctattgtttt cacaaggcaa aatgtacggc tatctggata
421 ccttgctaac catgctcgcc atgcttttaa aggttgcgat gaatagagcc caagtttgtt
481 tgatatctag ttccaaatct ggagaaagac atctctacct tattaaagtg tcaagagata
541 aaatatcaga caacaacgag caggaaacat caaattatga tgcaaaagca atatttgctg
601 tactcacgag tgtcttgccg aaagaagact ggtggaacct tctc
//