LOCUS BG086059 674 bp mRNA linear EST 12-MAY-2010 DEFINITION H3121C09-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3121C09 5', mRNA sequence. ACCESSION BG086059 VERSION BG086059.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 674) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG086059.1. Other_ESTs: H3121C09-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3121 row: C column: 09 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..674 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3121C09-5" /db_xref="taxon:10090" /clone="H3121C09" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 188 a 140 c 162 g 184 t ORIGIN 1 gacatggatc tgctgaatag taagaataac ttagtgatcg acacacctcg agttcggaaa 61 cagactcgac acttcagcac tttgaaagat gatgacctgg ttgagttttc tgatttggaa 121 agtgaagatg atgaacggcc acgttcccga agacatgacc ggcatcacac ctatgggcgc 181 actgactgct tccgggtaga aaagcatctc ctggtatatg ggtaagatgc agctttctgt 241 gagcagtagg gcatgtgcat gctgcctgac tttcaccatg gacaacgtgg ccaagaagca 301 ccacatgaaa gacacttggc ctgtgacaga gtttctgttt attttctact cttggaggag 361 atggcaattc tgggaaccag ggaaataaat atcactgaat tcgaatgtcc cccaaaatat 421 ttgtgttgct tacttttcct gttttgttag ttggggacgt tggagagata ttttgtctca 481 cggacgattt aagcggcgca tgactgaaag agatgttgag acaatatgcc gtgccatcct 541 cgtgtactgt cttctacact accgtgggga tgaaaacatc aaaagcttca tttgggactt 601 gattagccct gctgaaaatg gcaagacaaa agaattgcag aatcattcag gtaattatca 661 actcctacct cttc //