LOCUS BG085982 577 bp mRNA linear EST 12-MAY-2010 DEFINITION H3120D05-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3120D05 5', mRNA sequence. ACCESSION BG085982 VERSION BG085982.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 577) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG085982.1. Other_ESTs: H3120D05-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3120 row: D column: 05 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..577 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3120D05-5" /db_xref="taxon:10090" /clone="H3120D05" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 139 a 130 c 176 g 132 t ORIGIN 1 ggatagtcga gtccttcttg tcctggtcgt tgttcccgtc tgagtaccag ctccctatta 61 ccctgagggc gggcgagtgc agccgagaaa agaaggaggc ggaaaagaaa ttgtcccaga 121 tccctgcagt ctttccgtag gttgcggtac aacgccaggc aagagaagag gaaggaaatt 181 aaccagatcg gtgaaggtcg agttgagggt ctgctgtagc aggtggcact gcttgaacca 241 agggaggaac tttcccccgg atcagtagag atcagaaaga ctgttgggcg tggcataacc 301 cacaggaaag gaagaagggg caagctgctg ttttaaggag gtttacctct gtaagcaatt 361 actgtggcct tgaagtgact tgaagaagat cactctccac aggactgcac tcaggcccga 421 aggcctcttc ccttatcttg agtgactact ctatttcttg gtccctgccg ttgccaggga 481 cgattgcatg ggctacgcca ggaaagtagg ctgggtgact gcaggtctgg tgattggggc 541 tggagcctgc tattgcattt acagattgac ccgagga //