LOCUS BG085905 710 bp mRNA linear EST 12-MAY-2010 DEFINITION H3119E03-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3119E03 5', mRNA sequence. ACCESSION BG085905 VERSION BG085905.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 710) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG085905.1. Other_ESTs: H3119E03-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3119 row: E column: 03 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..710 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3119E03-5" /db_xref="taxon:10090" /clone="H3119E03" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 197 a 153 c 159 g 201 t ORIGIN 1 ctttcactgt cattctggtt ataaagtcca gtgctttctt gccttggtaa tatcatgagc 61 ttaagacatg tatgtcagta tcacagtgca ggtgttgtta gcataattca ccataattca 121 tggttctaca tctggtcctt tttctgtcaa aaaaaaaaaa agatatgtca tttaaatgta 181 tggttagcat cactccaatg ctgatgagca tttgagtgtg tgtgcagtaa tgtgttctgt 241 gttaatttag caaacattat tgtgcattgg ccacaactaa gtgcagtgaa ggcagcagaa 301 gtcagtccca ccagaaagca gttgtgtctg gttacattta agctctgtgg tgagcacaga 361 aattctgcct gacagagcag gatctctgac ttcctccatt gtgaccgtca ccaagaaagg 421 accagagctc ctgactagag acagcatcct tccgggtccc tgcagcttcc ttctggagcg 481 tgcagcatct tagaacccag tgcggtctag atccattctc acgtggtaga ttacttacat 541 gccgtctcca caggattggt tagaacttaa agaacagtga ggcccagcca gaaagccacg 601 gtccacactt acagtctgac acttacacac cctgaaagtc aggtcctttt gtgaagtgaa 661 atactgttgt gtgaagaggg cgatggaatt tagccaacag aattgaaaag //