LOCUS BG085904 706 bp mRNA linear EST 12-MAY-2010
DEFINITION H3119E02-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3119E02 5', mRNA sequence.
ACCESSION BG085904
VERSION BG085904.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 706)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG085904.1.
Other_ESTs: H3119E02-3
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3119 row: E column: 02
Seq primer: -21M13 Reverse
POLYA=No.
FEATURES Location/Qualifiers
source 1..706
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3119E02-5"
/db_xref="taxon:10090"
/clone="H3119E02"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 230 a 147 c 133 g 196 t
ORIGIN
1 ggatgaggaa cgggccttga gagcaaaacc caaagaagcc gcacagggac atgagagccc
61 atgggggagc ctctaagtca cgagtaaatt ctcagttctc tacagtaaac tataaagatg
121 agcttcagtc attaggagag acgcacgggg cgctggggat gtcgctcagt ggagagtgct
181 tgcctgacat gccgaaggtt ctaggtacaa catccccaga accattagaa aataaaaata
241 aaaggaaact atcagaaagg aacacaagtt tactacgtaa ttcctaaatt cccagcccca
301 cccccacccc cgacctgcaa ttgctgcttt ttcctggaag aacacataat gatcttataa
361 taaatacgca ttctaggata ttctctgtct tcctaaccta tacttccaaa gtgttaatgc
421 cagattaatt ggcctgcaat atatgttacc cttgagcctt ataatattgt ctgatgtata
481 aggcaattga ttttgtttaa ttcactgaca ctccttaacc cacctgtcag ctgcctgaga
541 gcaaagatca tggtttatta tttttaatat cttttgctta ttcagtatct cctgttatgc
601 agtatttaac tggtggtaat agtaaaggaa aaagcttaaa agataataaa acagtaattt
661 tccaattcaa ttttaaaaat agcattactg aaatatccag taagca
//