LOCUS BG085699 644 bp mRNA linear EST 12-MAY-2010 DEFINITION H3116E02-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3116E02 5', mRNA sequence. ACCESSION BG085699 VERSION BG085699.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 644) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG085699.1. Other_ESTs: H3116E02-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3116 row: E column: 02 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..644 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3116E02-5" /db_xref="taxon:10090" /clone="H3116E02" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 132 a 169 c 158 g 185 t ORIGIN 1 gccctctaca cagtcggcta cagcacctcc ctcgtcaccc tcaccactgc catggtcatc 61 ttgtgccgct tccggaagct gcactgtacc cgtaacttca tccacatgaa cctgtttgta 121 tccttcatgc tgagagctat ctctgtcttc atcaaagact ggatcttgta tgccgagcag 181 gacagcagtc attgcttcgt ttccaccgtg gaatgcaaag ctgtcatggt tttctttcac 241 tactgcgtgg tgtccaacta cttctggctg ttcattgaag gcctatacct ctttacactg 301 ctggtggaga ccttcttccc tgagaggaga tatttctact ggtatgccat cattggctgg 361 gggacaccta ctgtgtgtgt aactgtgtgg gctgtgctga ggctctactt tgatgatgcg 421 ggatgctggg atatgaatga cagcacagct ctgtggtggg tgatcaaagg ccctgtagtt 481 ggctctataa tggttaactt tgtgcttttc atcggcatca tcatcatcct tgtgcagaag 541 ctgcagtccc cagacatggg aggcaatgag tcgagcatct acttctgcgt gcagaaatgc 601 tactgcaagc cacagcgggc tcagcagcac tcttgcaaga tgtc //