LOCUS BG085654 681 bp mRNA linear EST 12-MAY-2010 DEFINITION H3115H10-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3115H10 5', mRNA sequence. ACCESSION BG085654 VERSION BG085654.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 681) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG085654.1. Other_ESTs: H3115H10-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3115 row: H column: 10 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..681 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3115H10-5" /db_xref="taxon:10090" /clone="H3115H10" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 183 a 139 c 179 g 180 t ORIGIN 1 ccatgttctc gcgggcgagc gttgtcgggc tgtcggcctg cgccgtgcag ccgcaatgga 61 tccaagttcg aaacaaggca actccgaaag atattaccag gagactgaag tccatcaaaa 121 acatccagaa aattaccaag tctatgaaga tggtggcagc tgcaaagtat gcccgggctg 181 agcgggagct gaagcctgcc cgagtgtatg ggacaggttc tttggctctg tatgagaagg 241 ctgatattaa ggcacctgag gacaagaaga agcacctcat tattggtgtg tcctcagata 301 gagggctttg tggtgctatt cactcctcag tggctaaaca gatgaagaat gaagtggctg 361 ccctcacagc agctgggaaa gaagttatga ttgttggagt tggtgaaaaa atcaagggca 421 tactttatag gactcattct gatcagtttt tggtgtcatt caaagatgtg ggacggaagc 481 ctcctacttt tggagatgca tcagtcattg cccttgagtt gttaaattct ggatatgaat 541 ttgatgaagg ctctatcatt tttaatcagt tcaagtctgt tatctcctac aagacagaag 601 agaagcccat cttctctctg aataccattg cgactgctga gaccatgagc atctatgatg 661 acattgatgc tgatgtgctg c //