LOCUS BG085547 602 bp mRNA linear EST 12-MAY-2010 DEFINITION H3114F01-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3114F01 5', mRNA sequence. ACCESSION BG085547 VERSION BG085547.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 602) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG085547.1. Other_ESTs: H3114F01-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3114 row: F column: 01 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..602 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3114F01-5" /db_xref="taxon:10090" /clone="H3114F01" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 182 a 131 c 122 g 167 t ORIGIN 1 ggaattgaag aggaagagat tcttccagga ttcatccttt gtgatcttaa taatctttgt 61 cattctggac gcacatttga tgctcagata gtgattatag agcacaagtc catcatctgc 121 ccagggtata atgcggtgct gcatattcat acctgtatcg aagaagtcga aataacagcc 181 ttagtctgct tggtagacaa gaaatcagga gagaaaagta agactcgacc ccgttttgta 241 aaacaagatc aagtatgcat tgcgcgttta aggacagcag gaaccatctg cctggagacc 301 tttaaggact tccctcagat gggacgtttt accttaagag atgagggtaa gaccattgca 361 attggaaaag ttctgaaact ggttccagag aaagactaag cattttcttg atgaccctgc 421 acaatactgt gaggaaaatt gactgcaaaa gcctacttca caccgccttc tcttactttc 481 tgcccattga caaacctctc cccatacttt gcaaagaaaa ttcacagcaa aagtccacat 541 tatgtcagct ttctcatatt gagagctctg ctatgccact gttgaatctt cccaagattt 601 tg //