LOCUS BG085050 547 bp mRNA linear EST 12-MAY-2010 DEFINITION H3108A10-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3108A10 5', mRNA sequence. ACCESSION BG085050 VERSION BG085050.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 547) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG085050.1. Other_ESTs: H3108A10-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3108 row: A column: 10 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..547 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3108A10-5" /db_xref="taxon:10090" /clone="H3108A10" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 174 a 110 c 149 g 114 t ORIGIN 1 cttggcggac gcggcggggt gggtagtgct gtcgtggtgg tggagtttgc tgagcatttc 61 ctagacctca gtaggcatgt cggcggcttt tcgcaaggca gccaagaccc ggcagcggga 121 acaccgagaa cgaagccagc ctggctttcg aaaacgcctg ggtttgctgg agaaaaagaa 181 agattacaaa cttcgtgcaa atgactatca taaaaaacaa gatttcctca gagctctccg 241 gaagaaggct cttgaaaaaa acccagatga attctactat aaaatgactc gggccaagct 301 ccaggatgga gttcacattt tcaaagagaa caaggaagaa gtgacagcag agcagctgaa 361 gctcatgaga actcaggaca tcaagtacat tgagatgaaa agagttgcag aggcgaagaa 421 aattgaaaga ttgaaatcag agctccatct gctggatttc caggggaagc aacagaagaa 481 gcacgtgttc ttttttgaca ccaaaaagga agttgaacgg ttttgatgtt gcaactcact 541 tgcagac //