LOCUS BG084542 593 bp mRNA linear EST 12-MAY-2010 DEFINITION H3102B07-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3102B07 5', mRNA sequence. ACCESSION BG084542 VERSION BG084542.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 593) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG084542.1. Other_ESTs: H3102B07-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3102 row: B column: 07 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..593 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3102B07-5" /db_xref="taxon:10090" /clone="H3102B07" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 109 a 175 c 97 g 212 t ORIGIN 1 ccctaaggag ttttgcgttg tagccccacc cactggccgc ctcacttcac acttgactcc 61 aaccgtcttt gcctgttccc ttctaccctg accgatttga gcatttcgac aagacaagtc 121 tcttgcttat atttctcctt ctaacctctc tccccggcac atttgctttt taaattgact 181 ttaacttggc cttttcttag tttactgcaa tctctggcca ctccttcatt cttctgccca 241 tggctccctt ctgctctaag cctagatttt tttttatttt attattatta ttattattat 301 tacttgtgtg tgtgtgtgga tcccacatcc tccaacagct ccaggggttg gaagctcctc 361 tctgtgctaa gagacgttgg gcttcttgct ttaatcctca cccttattta tctgaccctt 421 cacttttgat gctgatacct cccaacggcc ccaccttagc tctgtggcat tattatctcc 481 tctctgggac ctttcagccc ggcactccat acctctcgtg cccactcact ttaggcagct 541 tgcactattc ttaaatgaat gaagaatttc ctcatttgca ggtaggaggg gct //