LOCUS BG084477 594 bp mRNA linear EST 12-MAY-2010
DEFINITION H3101D04-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3101D04 5', mRNA sequence.
ACCESSION BG084477
VERSION BG084477.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 594)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG084477.1.
Other_ESTs: H3101D04-3
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3101 row: D column: 04
Seq primer: -21M13 Reverse
POLYA=No.
FEATURES Location/Qualifiers
source 1..594
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3101D04-5"
/db_xref="taxon:10090"
/clone="H3101D04"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 177 a 99 c 109 g 209 t
ORIGIN
1 gaggacacct gtctgcggaa taaagctgat catgttttgc tacagtttgc aggtgaacaa
61 aataaatctt agaaaatatg ctattgtttt tgtgttcttg ctgcaatgcc tttaccaaag
121 tggccttaaa tgtaatgagt agtgagttga gaacatcttg aactcttaaa gacttctagt
181 gactaacttt ttttaaaagg ccatgtagaa aatttattaa aactactagg actgctatat
241 tcaggaatgt gacagtggaa aagcatttaa atgtagcttg tcagattcac catgctactt
301 ctaatttctc tgcaacttct taattttgtg aaatttgtaa atatggagaa tttgcatgta
361 tgtgtattta caaatatttt ttaaagatct tgaattctca gactgcaaaa ggcctacttt
421 aactattttc ccctctgttt tcattgtctt tgaatgtatt ggaagcagac atgcattgac
481 tgtgacatgg tttcacctga ggttcccccc tcactttctg gacagactga tactatctat
541 caaacatttg tttctgttgg tttggggata aagaaaaagg aacccaatgt tacc
//