LOCUS BG084204 681 bp mRNA linear EST 12-MAY-2010 DEFINITION H3097F05-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3097F05 5', mRNA sequence. ACCESSION BG084204 VERSION BG084204.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 681) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG084204.1. Other_ESTs: H3097F05-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3097 row: F column: 05 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..681 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3097F05-5" /db_xref="taxon:10090" /clone="H3097F05" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 166 a 185 c 167 g 163 t ORIGIN 1 tacagccaga cccgaacctg gggagcaggg acagaagcca cacagaagtc ctcatcatcg 61 tttgcctccg tgaggccgct ggcctgtccc tggtgaggcc tggctgggga ggaaagcagt 121 gttgggtctt gcctgtcctt ctgaatcaaa gcagtagatt gtagatccta tggaaattca 181 gtgtctgatt tccggatgca gctttgcaat cgtttcccga cactgtgtgc actaagacct 241 ctttaaccag agtggctggc tggccactcc tacgtaaggc aataagtcac caggatgaga 301 ctagagatca tgtgactaca ctcagcaata atcactctca agacagactt caaaccccac 361 tgccatttct ccattaagcc agatgatcct gaggaccgac cactgcagaa agtcggctac 421 accttatttc agttgcaatg ttggccacat taccatggca acctggtcga agcacccacc 481 ctccttttag ttccagcatg tgctcctcag ctggggacct gcatctctag tgagcagaga 541 gtatcacggt ggttgctgtt gcttgttcca aaagaagagc tcggaaccct acagcagact 601 gctgggtttc cagcagggga gcaggaatga acctatctta tactccatct ggtttccatg 661 gtgacatcac agctgtgccc a //