LOCUS BG083914 589 bp mRNA linear EST 12-MAY-2010 DEFINITION H3093E12-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3093E12 5', mRNA sequence. ACCESSION BG083914 VERSION BG083914.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 589) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG083914.1. Other_ESTs: H3093E12-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3093 row: E column: 12 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..589 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3093E12-5" /db_xref="taxon:10090" /clone="H3093E12" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 141 a 147 c 145 g 156 t ORIGIN 1 ctcaccctgc agctgcattc catagagtct gtcagcattc cttccccagg tgttgtgaga 61 cacacccatg aagaagaagc agtctgatat atccttcctc atggcaggca gggagggtgg 121 agctgcatca gaaagcactg tcttccgtgt cattcctggt gaccgagatg gtttcggcag 181 atttctagtg gtcaggaatg taacagatgg gtgactttta gttgtcagaa ctgagagtta 241 tagttggagc catttttcca accctaagtc aattcgcaga cccagaactc tcctcagctg 301 gatggagagc catttactag agagtgtcct tgggagcaaa ggagtctctc agacttgtgc 361 accggttcct agaaaccgaa aataacaact tagcaactta ccgtggtcag gtagcagacg 421 gagtggcttc actcacatgt ggcttcactt tttcaaaagt tctccctcct ctgaatgggg 481 gtgagggcag gcaagcctct accacacggc tgctcaggtg acgctttcca aaccatagca 541 cactgtctcc ttcaccccgt gtttatgact tcttaggggg attttctta //