LOCUS BG083891 578 bp mRNA linear EST 12-MAY-2010 DEFINITION H3093C12-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3093C12 5', mRNA sequence. ACCESSION BG083891 VERSION BG083891.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 578) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG083891.1. Other_ESTs: H3093C12-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3093 row: C column: 12 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..578 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3093C12-5" /db_xref="taxon:10090" /clone="H3093C12" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 161 a 112 c 149 g 156 t ORIGIN 1 tatcacaata ccaaacttga caataatcta gattctgtgt ctctacttat agatcatgtt 61 tgtagtagga ggcagtttcc ctgttacact caacagatac ttacagagca ttgtaatgaa 121 gtgtggttct gtaaattttc taatgatggc actaaactag caacaggatc aaaggatacc 181 acagttatca tatggcaagt tgatccggat acgcacctgt taaaactgct taaaacgtta 241 gaaggacatg cgtatggtgt ctcttatata gcatggagtc cagatgacag ctatcttgtt 301 gcttgtggtc cagatgactg ctctgagctt tggctttgga atgtacagac gggagaatta 361 agaacaaaaa tgagccaatc tcatgaagac agtttgacca gtgtggcctg gaatccagat 421 gggaaacgct ttgtgacggg aggtcagcgt ggtcagttct accagtgtga cctggacgga 481 aatcttctgg actcctggga aggagtgcga gtacagtgcc tgtggtgctt gagtgacggg 541 aagactgtgc tggcttccga cacgcaccag agagtccg //