LOCUS BG083655 629 bp mRNA linear EST 26-JAN-2001 DEFINITION H3090F05-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3090F05 5', mRNA sequence. ACCESSION BG083655 VERSION BG083655.1 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 629) AUTHORS Kargul,G.J., Dudekula,D.B., Qian,Y., Lim,M.K., Jaradat,S.A., Tanaka,T.S., Carter,M.G. and Ko,M.S.H. TITLE Verification and initial annotation of NIA mouse 15K cDNA clone set JOURNAL Unpublished COMMENT Other_ESTs: H3090F05-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3090 row: F column: 05 Seq primer: -21M13 Reverse POLYA=Yes. FEATURES Location/Qualifiers source 1..629 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3090F05-5" /db_xref="taxon:10090" /clone="H3090F05" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 200 a 130 c 178 g 121 t ORIGIN 1 ttttttttta aagatagggt ttcaggtgtg tctgacatac atgaaaccct atctttaaaa 61 aaaaaccaaa aaacaaaaaa cctaagaaag taaccaatga tggaagtaag gcctccctct 121 tgggccagga ggaactatgc cctgcctctc tgaggaggta tggacccttc tagaatctga 181 gctcaccctc aagctctgcg ttgaagctgc tcacaggggc ccatggctga gacccagcaa 241 tcaggaagtc gaggagtgag aggccaccct gggcttcagt gtgagagtgt ctctcaaaga 301 gaaaaggaag gaaaaaagag agggactgaa agcaaagggg agagagggac tagaagagga 361 gaagctatga tccttaaagt agaagccagg ccaatggaag gactgcagag ctgcagaggg 421 agacatggca agagtctcta ttggcacaag ctatcaaggg agtaggtctg aagaagtggg 481 tggcacctcc tggagaagat gaggaaggcc agggtaaaga ctgggaaact tctccaggac 541 cctgggacca gagcatcctt gcagcattga gatcccaagt cagcacaaga cttgtgatct 601 aaagtgttat tagacgcgtt ctcacgccc //