LOCUS BG083643 540 bp mRNA linear EST 26-JAN-2001
DEFINITION H3090E04-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3090E04 5', mRNA sequence.
ACCESSION BG083643
VERSION BG083643.1
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 540)
AUTHORS Kargul,G.J., Dudekula,D.B., Qian,Y., Lim,M.K., Jaradat,S.A.,
Tanaka,T.S., Carter,M.G. and Ko,M.S.H.
TITLE Verification and initial annotation of NIA mouse 15K cDNA clone set
JOURNAL Unpublished
COMMENT Other_ESTs: H3090E04-3
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3090 row: E column: 04
Seq primer: -21M13 Reverse
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..540
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3090E04-5"
/db_xref="taxon:10090"
/clone="H3090E04"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 129 a 109 c 120 g 182 t
ORIGIN
1 tttttttttt ttttcatttt aaaggatctc tccttcagca tgaggttttt ggtgctttgc
61 ttttggacct ctctttattg agtcattttc tagtttacaa agacttagga gtacaaaaac
121 gtgggagtac agagagatgt tacatctttt ctgtttttct cctttgagtc tactgagcag
181 accatttttc ctgtgtggtg catgatgagg agtcgcagtc accgagggta ctccgaaccc
241 ttggctgatg tcagcggaca gctgcaggtg ggaacgggtc ctcagtgatg gttacagatg
301 ccagatgatt ctagaacgct gaatcctgaa ttaagaatct agatcagtgt tctcaacctt
361 agtgatgtgt tgtgttggcc cccaaccata acatgatttt cattcctact tcataactgc
421 aactttacta ctgttattaa tgatcatgta agtatctggt gggctggata tttgatatct
481 gacccctgtg gataggtcat tcaacctgcc caaggggtca agacccacaa gttaacacag
//