LOCUS BG083613 665 bp mRNA linear EST 26-JAN-2001 DEFINITION H3090B04-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3090B04 5', mRNA sequence. ACCESSION BG083613 VERSION BG083613.1 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 665) AUTHORS Kargul,G.J., Dudekula,D.B., Qian,Y., Lim,M.K., Jaradat,S.A., Tanaka,T.S., Carter,M.G. and Ko,M.S.H. TITLE Verification and initial annotation of NIA mouse 15K cDNA clone set JOURNAL Unpublished COMMENT Other_ESTs: H3090B04-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3090 row: B column: 04 Seq primer: -21M13 Reverse POLYA=Yes. FEATURES Location/Qualifiers source 1..665 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3090B04-5" /db_xref="taxon:10090" /clone="H3090B04" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 244 a 109 c 101 g 211 t ORIGIN 1 tttttttttt tttttggttg ttaaacagct taaattttat tgacctccca gtttttaaaa 61 aagttaaagg tcacacatct aagttgatgt tctatgtaca gatcgtgcag aaagagtatg 121 aagagataca aattagtcat gccaaaaaga ttgactagag tacagagcca tcttcataaa 181 tacatataaa attcttatga agatagaggg gattgccagg ttttctgaga cactttccaa 241 agttattcaa ggcacaaaat atacaccatt gtgaatatac atattctaga ctttgtgcct 301 ctgatacagc gcagagattt ggcttcatga ctattataaa actaaatata ctgaatgaga 361 ttgtttcttg ggtgaaaaag ccagacctat aaacatcaag tagataatta ctccagaata 421 tggagaatac aaatacaagc actttatttt aaaaagcaaa cacaaaagat catgtaaatc 481 caagtgttaa ctgtgtcaag cttccatttt ggatcattta gaatctacac aagtttgttc 541 ccagctcatc catcactagt ctctactaag gaaaatgatt ctaaaaatta acatggtttt 601 cagtatttaa aatctcacta tttatataaa agcctagaga gaccaataat atccaatagc 661 ttcaa //