LOCUS BG083408 633 bp mRNA linear EST 12-MAY-2010 DEFINITION H3087H08-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3087H08 5', mRNA sequence. ACCESSION BG083408 VERSION BG083408.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 633) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG083408.1. Other_ESTs: H3087H08-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3087 row: H column: 08 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..633 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3087H08-5" /db_xref="taxon:10090" /clone="H3087H08" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 145 a 142 c 156 g 190 t ORIGIN 1 gtttgtttta agtacatggt tgatcttatg cctgggttac agacctatgt actggagcca 61 cagaacagct tctttggtga aaaggagtct ggcgtgcaaa gggcttccat ggaatagcag 121 ctctccagct ttggggcatc agagttcctt agcgcagcag ctcctccacc ccgctcccac 181 attgctgagt cagtcagggg taggtctgga aactgacatg tctcacatgt tcccagctgc 241 tgccagcggc cagtgggaga cacactgcca cagtagatga ctgtggattg tttgttttgt 301 tttgtttttc taatgtattg tcagaaccta tttgacgcat tgttgaggct ttctaattgg 361 tccctctatt gggcgaagca caaatgttgc cttgtaaatt ccagtttggg aaaagaggtt 421 tctagggcat ttatgaagca agaaacagat atgtgttgtt tccttccagc ttgcctctgc 481 ccttgacctt cccctgctac gtattaatca agcaaatagc cctgatctcc tcagtgtgtc 541 tcagtattat tcaggagaat tggtgtcata cgtgagaaag gtaaggactg cctctgatag 601 ctgcttcctt aaataaacag tgccctggcg gag //