LOCUS BG083368 630 bp mRNA linear EST 12-MAY-2010 DEFINITION H3087E03-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3087E03 5', mRNA sequence. ACCESSION BG083368 VERSION BG083368.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 630) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG083368.1. Other_ESTs: H3087E03-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3087 row: E column: 03 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..630 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3087E03-5" /db_xref="taxon:10090" /clone="H3087E03" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 182 a 148 c 146 g 154 t ORIGIN 1 tggctacagg tggcacctga atgaagggcc tgaaaacagg ggaacctact gagaaacggt 61 gtcttgggtg gagctccata gaaaacacaa gaaaagtgaa agtatccatg aagcatgaaa 121 gaaaatggag ctcaatgact gcctgaaaga ttggttcatc aagtggacat ggatcctggg 181 ccttgtctta tcaacctgga cagagacatt tgtcttggct acagttgcag gttctcatgg 241 gaacttagct gttttggctg cctacgctga ccttgttcta caacattctc cagctgaaga 301 aggggatgag tgtggatcct ctcgtgactt cgaaaagatg tggagcgact gagagctttc 361 ccacactgct tacactgggc taaggaattt cactcttacc aatagaagcc aggttatcaa 421 cggtttcctg catcacatct tggtacagtt ccttctgaga atcgtccagc agagcccact 481 cctcatgggt gaagttcaca gctacatcct caaaagccac tgagtcctaa aacatcccat 541 gaaagaagaa tggcgaacat gacaacactc acctcatgaa gagactcaca tgattctgct 601 tcctacaaca cgtctataac ttggtcctta //